产品名称：avivasysbio/Hsp40 Antibody (OASE00326)/25ul/OASE00326

产品编号：OASE00326

市场价：¥3500.00

场地：美国(厂家直采)

产品分类：抗体类>一抗>功能性抗体>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$175.00

品牌： avivasysbio

公司：avivasysbio

公司分类：

商品介绍

Predicted Species Reactivity:

Human

Clonality:

Polyclonal

Host:

Rabbit

Application:

WB, IP

Additional Information:

Background Info: Detects an ~40kDa protein corresponding to the molecular mass of Hsp40 on SDS PAGE immunoblots. Also detects recombinant Hsp40.

:::

Scientific Background: DnaJ/Hsp40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, Hsp70s. Because the ATP hydrolysis is essential for the activity of Hsp70s, DnaJ/Hsp40 proteins actually determine the activity of Hsp70s by stabilizing their interaction with substrate proteins. DnaJ/Hsp40 proteins all contain the J domain through which they bind to Hsp70s.Hsp40, also known as HDJ1 (6), is a basic mammalian 40kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (2-5). Hsp 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli; an intracellular pattern similar to Hsc70/Hsp70, the mammalian homologues of the bacterial heat shock protein, DnaK (2).

:::

Certificate of Analysis: 0.5 ug/mL was sufficient for detection of Hsp40 in 20ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Reconstitution and Storage:

Store at -20C. Shipping in Blue Ice or 4C.

Replacement Item:

This antibody may replace item sc-114001 from Santa Cruz Biotechnology.

Immunogen:

Recombinant purified human hsp40

Purification:

Rabbit antiserum

Datasheets/Manuals:

Printable datasheet for OASE00326

Dilution:

1: 1500-2,000 (WB)

Target Reference:

1. Melville, M. W. et al. (1997) PNAS USA, 94: 97-102. 2. Hattori, H., Liu, Y-C., Tohnai, I., Ueda, M., Kaneda, T., Kobayashi, T., Tanabe, K., and Ohtsuka, K. (1992) Cell Structure and Function 17: 77-86.3. Ohtsuka, K. Masuda, A., Nakai, A., and Nagata, K. (1990) Biochem. Biophys. Res. Commun. 166: 642-647.4. Bardwell, J.C.A., Tilly, K., Craig, E., King, J., Zylicz, M. and Georgopoulos, C. (1986) J. Biol. Chem. 261: 1782-1785.5. Ohku, M., Tamura, F., Nishimura, S., and Uchida, H. (1986) J. Biol. Chem. 261: 1778-1781.6. Ohtsuka, K. (1993) Biochem. Biophys. Res. Commun. 197: 235-240.

Storage Buffer:

Rabbit antiserum

Gene Symbol:

DNAJB1

Official Gene Full Name:

DnaJ (Hsp40) homolog, subfamily B, member 1

NCBI Gene Id:

3337

Swissprot Id:

P25685

Protein Accession #:

NP_006136.1

Tissue Tool:

Find tissues and cell lines supported by DNA array analysis to express DNAJB1.

RNA Seq:

Find tissues and cell lines supported by RNA-seq analysis to express DNAJB1.

品牌介绍

avivasysbio英杰华系统生物学

关键目标家族和独特内容的抗体

我们丰富的抗体目录

Aviva Systems Biology为我们的客户提供了大量用于研究和诊断应用的高质量抗体。英杰华目前提供针对7,000多种不同蛋白质靶标的抗体，这是因为我们的高通量抗体生产每月会释放200多种新抗体。Aviva的重点是针对转录，表观遗传学和细胞信号传导领域关键靶标的抗体。Aviva的抗体库包含针对许多目标的独特抗体含量，这些目标是其他地方无法买到的。我们将重点放在面向家庭的抗体生产上，从而产生了针对大多数关键蛋白质家族成员的抗体，例如转录因子，转录调节剂，RNA结合蛋白，离子通道和细胞膜靶蛋白。