产品名称：avivasysbio/SOX9 ELISA Kit (Dog) (OKEH07411)/96 Wells/OKEH07411

产品编号：OKEH07411

市场价：¥17380.00

场地：美国(厂家直采)

产品分类：试剂盒>ELISA试剂盒>特色ELISA试剂盒>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$869.00

品牌： avivasysbio

公司：avivasysbio

公司分类：

商品介绍

Predicted Species Reactivity:

Dog

Application:

ELISA

Kit Detection Method:

Colorimetric, OD450 nm

Kit Principle:

Aviva Systems Biology SOX9 ELISA Kit (Dog) (OKEH07411) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SOX9 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SOX9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SOX9 captured in well.

Kit Range:

0.156 - 10ng/mL

Kit Recovery:

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97% (n = 20)

Kit Reproducibility:

Mean Intra-assay CV%: <=3.0% (n="">Mean Inter-assay CV%: <=9.7% (n="">

Kit Component:

Component	Amount
SOX9 Microplate	96 Wells (12 x 8 Well strips)
SOX9 Lyophilized Standard	2
100X Biotinylated SOX9 Detector Antibody	1 x 120 uL
100X Avidin-HRP Conjugate	1 x 120 uL
Sample Diluent	1 x 20 mL
Detector Antibody Diluent	1 x 12 mL
Conjugate Diluent	1 x 12 mL
25X Wash Buffer	1 x 30 mL
TMB Substrate	1 x 10 mL
Stop Solution	1 x 10 mL

Additional Information:

Function: Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.

:::

Subcellular Location: Nucleus

Reconstitution and Storage:

Store as indicated in product manual.

Sensitivity:

0.087ng/mL

Datasheets/Manuals:

Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.

Specificity:

Natural and recombinant Dog Transcription factor SOX-9

Assay Info:

Assay Methodology: Quantitative Sandwich ELISA

Gene Symbol:

SOX9

Official Gene Full Name:

SRY-box transcription factor 9

NCBI Gene Id:

403464

Protein Name:

transcription factor SOX-9

Swissprot Id:

Q7YRJ7

Protein Accession #:

NP_001002978.1

Nucleotide Accession #:

NM_001002978.1

Tissue Tool:

Find tissues and cell lines supported by DNA array analysis to express SOX9.

RNA Seq:

Find tissues and cell lines supported by RNA-seq analysis to express SOX9.

品牌介绍

avivasysbio英杰华系统生物学

关键目标家族和独特内容的抗体

我们丰富的抗体目录

Aviva Systems Biology为我们的客户提供了大量用于研究和诊断应用的高质量抗体。英杰华目前提供针对7,000多种不同蛋白质靶标的抗体，这是因为我们的高通量抗体生产每月会释放200多种新抗体。Aviva的重点是针对转录，表观遗传学和细胞信号传导领域关键靶标的抗体。Aviva的抗体库包含针对许多目标的独特抗体含量，这些目标是其他地方无法买到的。我们将重点放在面向家庭的抗体生产上，从而产生了针对大多数关键蛋白质家族成员的抗体，例如转录因子，转录调节剂，RNA结合蛋白，离子通道和细胞膜靶蛋白。