产品名称：avivasysbio/HTR1B ELISA Kit (Pig) (OKEH07572)/96 Wells/OKEH07572

产品编号：OKEH07572

市场价：¥15980.00

场地：美国(厂家直采)

产品分类：试剂盒>ELISA试剂盒>夹心法ELISA>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$799.00

品牌： avivasysbio

公司：avivasysbio

公司分类：

商品介绍

PredictedSpeciesReactivity:

Pig

Application:

ELISA

KitDetectionMethod:

Colorimetric,OD450nm

KitLinearity:

Sample	1:2	1:4	1:8	1:16
serum(n=5)	82-94%(n=20)	107-117%(n=20)	82-92%(n=20)	110-120%(n=20)
EDTAplasma(n=5)	93-102%(n=20)	96-106%(n=20)	83-93%(n=20)	100-113%(n=20)
heparinplasma(n=5)	97-109%(n=20)	84-92%(n=20)	93-104%(n=20)	99-112%(n=20)

KitPrinciple:

AvivaSystemsBIOLOGyHTR1BELISAKit(Pig)(OKEH07572)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyspecificforHTR1Bhasbeenpre-coatedontoa96-wellplate(12x8WellStrips)andblocked.Standardsortestsamplesareaddedtothewells,incubatedandremoved.AbiotinylateddetectorantibodyspecificforHTR1Bisadded,incubatedandfollowedbywashing.Avidin-PeroxidaseConjugateisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangesyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsampleHTR1Bcapturedinwell.

KitRecovery:

Meanrecoverywhenspikingintosamplematricesatconcentrationswithinthedynamicrange:97%(n=20)

KitReproducibility:

MeanIntra-assayCV%:<=4.2%(n="">MeanInter-assayCV%:<=7.3%(n="">

KitComponent:

Component	Amount
HTR1BMicroplate	96Wells(12x8Wellstrips)
HTR1BLyophilizedStandard	2
100XBiotinylatedHTR1BDetectorAntibody	1x120uL
100XAvidin-HRPConjugate	1x120uL
SampleDiluent	1x20mL
DetectorAntibodyDiluent	1x12mL
ConjugateDiluent	1x12mL
25XWashBuffer	1x30mL
TMBSubstrate	1x10mL
StopSolution	1x10mL

AdditionalInformation:

Function:G-proteincoupledreceptorfor5-hydroxytryptamine(SEROtonin).Alsofunctionsasareceptorforvariousalkaloidsandpsychoactivesubstances.Ligandbindingcausesaconformationchangethattriggerssignalingviaguaninenucleotide-bindingproteins(Gproteins)andmodulatestheactivityofdown-streameffectors,suchasadenylatecyclase.Signalinginhibitsadenylatecyclaseactivity.ArrestinfamilymembersinhibitsignalingviaGproteinsandmediateactivationofalternativesignalingpathways.Regulatesthereleaseof5-hydroxytryptamine,dopamineandacetylcholineinthebrain,andtherebyaffectsneuralactivity,nociceptiveprocessing,painperception,moodandbehavior.Besides,playsaroleinvasoconstrictionofcerebralarteries.

:::

SubcellularLocation:CellmembraneMulti-passmembraneprotein

:::

Unigene:Ssc.16375SMR:P79399String:9823.ENSSSCP00000004826

:::

Pfam:PF00001

:::

Pfam:PF00001

ReconstitutionandStorage:

Storeasindicatedinproductmanual.

Datasheets/Manuals:

Clickheretodownloadproductmanual.Asvariationbetweenlotsmayoccur,alwaysreferencethelot-specificmanualreceivedwitheachkit.

Specificity:

NaturalandrecombinantPig5-hydroxytryptaminereceptor1B

AssayInfo:

AssayMethodology:QuantitativeSandwichELISA

GeneSymbol:

HTR1B

AliasSymbols:

5-HT-1B,Serotoninreceptor1B

ProteinName:

5-hydroxytryptaminereceptor1B

SwissprotId:

P79399

品牌介绍

avivasysbio英杰华系统生物学

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