货号:OPCD06400
英文名:PRKAA2
保存条件:ReconstituteinPBSorothers.
Storeat2-8Cforonemonth.Aliquotandstoreat-80Cfor12months.Avoidrepeatedfreeze/thawcycles.
规格:10ug
NCBIGeneId:5563
SwissprotId:P54646
CatalyticsubunitofAMP-activatedproteinkinase(AMPK),anenergysensorproteinkinasethatplaysakeyroleinregulatingcellularenergymetabolism.InresponsetoreductionofintracellularATPlevels,AMPKactivatesenergy-producingpathwaysandinhibitsenergy-consumingprocesses:inhibitsprotein,carbohydrateandlipidbiosynthesis,aswellascellgrowthandproliferation.AMPKactsviadirectphosphorylationofmetabolicenzymes,andbylonger-termeffectsviaphosphorylationoftranscriptionregulators.Alsoactsasaregulatorofcellularpolaritybyremodelingtheactincytoskeleton;probablybyindirectlyactivatingmyosin.RegulateslipidsynthesisbyphosphorylatingandinactivatinglipidmetabolicenzymessuchasACACA,ACACB,GYS1,HMGCRandLIPE;regulatesfattyacidandcholesterolsynthesisbyphosphorylatingacetyl-CoAcarboxylase(ACACAandACACB)andhormone-sensitivelipase(LIPE)enzymes,respectively.Regulatesinsulin-signalingandglycolysisbyphosphorylatingIRS1,PFKFB2andPFKFB3.Involvedininsulinreceptor/INSRinternalization.AMPKstimulatesglucoseuptakeinmusclebyincreasingthetranslocationoftheglucosetransporterSLC2A4/GLUT4totheplasmamembrane,possiblybymediatingphosphorylationofTBC1D4/AS160.RegulatestranscriptionandchromatinstructurebyphosphorylatingtranscriptionregulatorsinvolvedinenergymetabolismsuchasCRTC2/TORC2,FOXO3,histoneH2B,HDAC5,MEF2C,MLXIPL/ChREBP,EP300,HNF4A,p53/TP53,SREBF1,SREBF2andPPARGC1A.ActsasakeyregulatorofglucosehomeostasisinliverbyphosphorylatingCRTC2/TORC2,leadingtoCRTC2/TORC2sequestrationinthecytoplasm.Inresponsetostress,phosphorylatesSer-36ofhistoneH2B(H2BS36ph),leadingtopromotetranscription.ActsasakeyregulatorofcellgrowthandproliferationbyphosphorylatingTSC2,RPTORandATG1/ULK1:inresponsetonutrientlimitation,negativelyregulatesthemTORC1complexbyphosphorylatingRPTORcomponentofthemTORC1complexandbyphosphorylatingandactivatingTSC2.Inresponsetonutrientlimitation,promotesautophagybyphosphorylatingandactivatingATG1/ULK1.AMPKalsoactsasaregulatorofcircadianrhythmbymediatingphosphorylationofCRY1,leadingtodestabilizeit.MayregulatetheWntsignalingpathwaybyphosphorylatingCTNNB1,leadingtostabilizeit.AlsophosphorylatesCFTR,EEF2K,KLC1,NOS3andSLC12A1.Playsanimportantroleinthedifferentialregulationofpro-autophagy(composedofPIK3C3,BECN1,PIK3R4andUVRAGorATG14)andnon-autophagy(composedofPIK3C3,BECN1andPIK3R4)complexes,inresponsetoglucosestarvation.Caninhibitthenon-autophagycomplexbyphosphorylatingPIK3C3andcanactivatethepro-autophagycomplexbyphosphorylatingBECN1.-Seemoreat:http://www.avivasysbio.com/sd/tds/html_datasheet.php?sku=OPCD06400

PRKAA2 Recombinant Protein (OPCD06400) using SDS-PAGE

SDS-PAGE

PRKAA2RecombinantProtein(OPCD06400)usingSDS-PAGE

温馨提示:不可用于临床治疗。