产品名称：avivasysbio/Human CD8 Antibody : APC-Cy7 (OATA00117)/OATA00117

产品编号：OATA00117

市场价：¥0.00

场地：美国(厂家直采)

产品分类：抗体类>二抗>抗人>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： avivasysbio

公司：avivasysbio

公司分类：

商品介绍

Tested Species Reactivity:

Human

Predicted Species Reactivity:

African Green, Baboon, Chimpanzee, Cynomolgus, Rhesus, Pigtailed Macaque, Sooty Mangabey

Product Format:

Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2

Clonality:

Monoclonal

Clone:

SK1

Isotype:

Mouse IgG1, kappa

Host:

Mouse

Conjugation:

APC: Allophycocyanin. CY7: Cyanine 7

Application:

Additional Information:

Description:

The SK1 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.

The SK1 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. It is cross-reactive with CD8 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus. If used together with an alternative Anti-Human CD8a clone, RPA-T8, the SK1 antibody will not block binding of RPA-T8 to CD8a.

:::

Excitation Laser: Red (633-640 nm)

:::

Emission: 785 nm

Reconstitution and Storage:

2-8C protected from light

Datasheets/Manuals:

Printable datasheet for OATA00117

Specificity:

CD8

Application Info:

This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.

Target Reference:

Campanelli R, Palermo B, Garbelli S, Mantovani S, Lucchi P, Necker A, Lantelme E and Giachino C. 2002. Int Immunol. 14(1): 39-44. (Flow Cytometry)

Walter S, Herrgen L, Schoor O, Jung G, Wernet D, Buhring H-J, Rammensee H-G and Stevanovic S. 2003. J. Immunol. 171(10): 4974-4978. (Flow Cytometry)

Cervasi B, Paiardinin M, Serafini S, Fraternale A, Menotta M, Engram J, Lawson B, Staprans I, Piedimonte G, Perno CF, Silvestri G and Magnani M. 2006. J. Virol. 80(21): 10335-10345. (Flow Cytometry - Sooty Mangabeys)

Verstrepen BE, Verschoor EJ, Fagrouch ZC, Mooij P, de Groot NG, Bontrop RE, Bogers WM, Heeney JL and Koopman G. 2014. PLoS ONE 9(4): e95103. doi:10.1371/journal.pone.0095103. (Flow Cytometry - Chimpanzee)

Permar SR, Klumpp SA, Mansfield KG, Kim W-K, Gorgone DA, Lifton MA, Williams KC, Schmitz JE, Reimann KA, Axthelm MK, Polack FP, Griffin DE and Letvin NL. 2003. J. Virol. 77(7): 4396-4400. (Flow Cytometry - Rhesus)

Ahmed AFK, Ohtani H, Nio M, Fuanki N, Shimaoka S, Nagura H and Ohi R. 2001. J. Pathol. 193(3): 383-389. (Immunohistochemistry)

Bukowska-StrakovÃ¡ K, Baran J, Gawlicka M and Kowalczyk, D. 2006. Folia Histochemica et Cytobiologica 44(3): 179-183.

Gene Symbol:

CD8A

Official Gene Full Name:

CD8a molecule

Alias Symbols:

CD8, p32, Leu2

NCBI Gene Id:

925

Protein Name:

T-cell surface glycoprotein CD8 alpha chain

Description of Target:

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene.

Swissprot Id:

P01732

Protein Accession #:

NP_001139345.1

Nucleotide Accession #:

NM_001145873.1

Tissue Tool:

Find tissues and cell lines supported by DNA array analysis to express CD8A.

RNA Seq:

Find tissues and cell lines supported by RNA-seq analysis to express CD8A.

品牌介绍

avivasysbio英杰华系统生物学

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Aviva Systems Biology为我们的客户提供了大量用于研究和诊断应用的高质量抗体。英杰华目前提供针对7,000多种不同蛋白质靶标的抗体，这是因为我们的高通量抗体生产每月会释放200多种新抗体。Aviva的重点是针对转录，表观遗传学和细胞信号传导领域关键靶标的抗体。Aviva的抗体库包含针对许多目标的独特抗体含量，这些目标是其他地方无法买到的。我们将重点放在面向家庭的抗体生产上，从而产生了针对大多数关键蛋白质家族成员的抗体，例如转录因子，转录调节剂，RNA结合蛋白，离子通道和细胞膜靶蛋白。