| Predicted Species Reactivity | Human |
|---|---|
| Product Format | Liquid |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Conjugation | Unconjugated |
| Application | WB |
| Reconstitution and Storage | Store at -20C. Avoid repeated freeze/thaw cycles. |
| Immunogen | A phospho-Serpecific peptide corresponding-Thro residues-Serurrounding-Thr514 of human PKC (pan) (gamma) |
| Purification | Affinity purified against immunogen |
| Datasheets/Manuals | Printable datasheet for anti-PRKCA (OAAN03120) |
| Preservative | 0.02% Sodium Azide |
| Target Post-Translational Modification | Phospho-Thr514 |
| Application Info | WB: 1:500 - 1:2,000 |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Gene Symbol | PRKCA |
|---|---|
| Official Gene Full Name | protein kinase C alpha |
| Alias Symbols | , protein kinase C alpha, Protein kinase C alpha type, PRKCA |
| NCBI Gene Id | 5578; 5579; 5580; 5581; 5582; 5583; 5588 |
| Protein Name | Protein kinase C alpha type |
| Description of Target | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. |
| Swissprot Id | P17252 |
| Protein Accession # | NP_002728.1 |
| Nucleotide Accession # | NM_002737.2 |
| Molecular Weight | 77 kDa |
| Tissue Tool | Find tissues and cell lines supported by DNA array analysis to express PRKCA.  |
| RNA Seq | Find tissues and cell lines supported by RNA-seq analysis to express PRKCA.  |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
- IHC Tips & Tricks
- ICC Tips & Tricks
- ELISA Tips & Tricks
- WB/IB Tips & Tricks
- IP Tips & Tricks
See our General FAQ page.
What is the species homology for "PRKCA Antibody (Phospho-Thr514) (OAAN03120)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
How long will it take to receive "PRKCA Antibody (Phospho-Thr514) (OAAN03120)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
What buffer format is "PRKCA Antibody (Phospho-Thr514) (OAAN03120)" provided in?
This item is provided in "Liquid".Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "PRKCA Antibody (Phospho-Thr514) (OAAN03120)"?
This target may also be called ", protein kinase C alpha, Protein kinase C alpha type, PRKCA" in publications.
What is the shipping cost for "PRKCA Antibody (Phospho-Thr514) (OAAN03120)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
What is the guarantee for "PRKCA Antibody (Phospho-Thr514) (OAAN03120)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "PRKCA Antibody (Phospho-Thr514) (OAAN03120)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "77 kDa".Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "PRKCA Antibody (Phospho-Thr514) (OAAN03120)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for "PRKCA"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for "PRKCA"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with "PRKCA"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with "PRKCA"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with "PRKCA"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with "PRKCA"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
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