产品名称：avivasysbio/Human CD11b Antibody : FITC (OATA00199)/OATA00199

产品编号：OATA00199

市场价：¥0.00

场地：美国(厂家直采)

产品分类：抗体类>二抗>抗人>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： avivasysbio

公司：avivasysbio

公司分类：

商品介绍

Tested Species Reactivity:

Human

Predicted Species Reactivity:

Baboon, Chimpanzee, Cynomolgus, Rhesus, Swine

Product Format:

Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2

Clonality:

Monoclonal

Clone:

ICRF44

Isotype:

Mouse IgG1, kappa

Host:

Mouse

Conjugation:

FITC: Fluorescein Isothiocyanate

Application:

Additional Information:

Description:

The ICRF44 antibody reacts with human CD11b, also known as integrin alpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrins are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin receptors consist of distinct combinations of integrin chains which are differentially expressed. Integrin alpha M (CD11b) assembles with Integrin beta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1, ICAM-2, ICAM-3 and ICAM-4 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.

The ICRF44 antibody is widely used as a marker for CD11b expression on macrophages, granulocytes, and subsets of NK cells. It is reported to be cross-reactive with a number of non-human species including Baboon, Chimpanzee, Cynomolgus, Rhesus and Swine.

:::

Excitation Laser: Blue (488 nm)

:::

Emission: 520 nm

Reconstitution and Storage:

2-8C protected from light

Datasheets/Manuals:

Printable datasheet for OATA00199

Specificity:

CD11b

Application Info:

This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.

Target Reference:

Feng C, Zhang L, Almulki L, Faez S, Whitford M, Hafezi-Moghadam A, and Cross AS. 2011. J. Leukoc. Biol. 90:313-321. (Immunoprecipitation)

Chang WLW and Barry PA. 2010. Proc. Natl. Acad. Sci. 107:22647-2652. (Flow cytometry - Rhesus macaque)

Jerke U, Rolle S, Dittmar G, Bayat B, Santoso S, Sporbert A, Luft F, and Kettritz R. 2010. J. Biol. Chem. 286:7070-7081. (in vitro blocking)

Moreau A, Hill M, Thebault P, Deschamps JY, Chiffoleau E, Chauveau C, Moullier P, Anegon I, Alliot-Licht B, and Cuturi MC. 2009. FASEB J. 23:3070-3077. (Flow cytometry - cynomolgus macaque)

Sengoku K, Takuma N, Miyamoto T, Horikawa M, and Ishikawa M. 2004. Hum. Reprod. 19: 639-644. (Immunofluorescence microscopy)

David A, Kacher Y, Specks U, and Aviram I. 2003. J. Leukoc. Biol. 74:551-557. (Western blot)Rezzonico R, Imbert V, Chicheportiche R, and Dayer J-M. 2001. Blood. 97: 2932-2940. (in vitro activation)

Gene Symbol:

ITGAM

Official Gene Full Name:

integrin subunit alpha M

Alias Symbols:

CR3A, MO1A, CD11B, MAC-1, MAC1A, SLEB6

NCBI Gene Id:

3684; 454069; 101866035

Protein Name:

integrin alpha-M

Description of Target:

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene.

Swissprot Id:

P11215

Protein Accession #:

NP_000623.2

Nucleotide Accession #:

NM_000632.3

Tissue Tool:

Find tissues and cell lines supported by DNA array analysis to express ITGAM.

RNA Seq:

Find tissues and cell lines supported by RNA-seq analysis to express ITGAM.

品牌介绍

avivasysbio英杰华系统生物学

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