产品名称：avivasysbio/Human/Mouse CD45R Antibody : APC-Cy7 (OATA00126)/OATA00126

产品编号：OATA00126

市场价：¥0.00

场地：美国(厂家直采)

产品分类：抗体类>二抗>抗大鼠>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： avivasysbio

公司：avivasysbio

公司分类：

商品介绍

Tested Species Reactivity:

Human, Mouse

Predicted Species Reactivity:

Feline

Product Format:

Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2

Clonality:

Monoclonal

Clone:

RA3-6B2

Isotype:

Rat IgG2a, kappa

Host:

Rat

Conjugation:

APC: Allophycocyanin. CY7: Cyanine 7

Application:

Additional Information:

Description:

The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.

The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets and NK cell subsets in human and mouse. This antibody has also been reported as cross-reactive with feline CD45R/B220.

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Excitation Laser: Red (633-640 nm)

:::

Emission: 785 nm

Reconstitution and Storage:

2-8C protected from light

Datasheets/Manuals:

Printable datasheet for OATA00126

Specificity:

CD45R (B220)

Application Info:

This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Please refer to the figure legend for the optimal concentration used to stain the tissue shown. We recommend titrating the antibody under your specific conditions to determine the optimal concentration of antibody needed in your experimental system.

Target Reference:

Willinger T and Flavell RA. 2012. Proc. Natl. Acad. Sci. 109:8670-8675. (Flow cytometry)

Meredith MM, Liu K, Darrasse-Jeze G, Kamphorst AO, Schreiber HA, Guermonprez P, Idoyaga J, Cheong C, Yao K-H, Niec RE, and Nussenzweig MC. 2012. J. Exp. Med. 209: 1153-1165. (Immunofluorescence microscopy: acetone-fixed frozen tissue)

Becker-Herman A, Meyer-Bahlburg A, Schwartz MA, Jackson SW, Hudkins KL, Liu C, Sather BD, Khim S, Liggitt D, Song W, Silverman GJ, Alpers CE and Rawlings DJ. 2011. J. Exp. Med. 208:2033-2042. (Immunofluorescence microscopy - OCT embedded frozen tissue)

Bertossi A, Aichinger M, Sansonetti P, Lech , Neff F, Pal M, Wunderlich FT, Anders H, Klein L, and Schmidt-Supprian M. 2011. J. Exp. Med. 208:1749-1756. (Immunofluorescence microscopy)

De Clercq S, Gembarska A, Denecker G, Maetens M, Naessens M, Haigh K, Haigh JJ, and Marine J-C. 2010. Mol. Cell. Biol. 30:5394-5405. (Western Blot)

Nutt SL, Metcalf D, D"™Amico A, Polli M, and Wu L. 2005. J. Exp. Med. 201:221-231. (Immunomagnetic bead depletion)

Cappione AJ, Pugh-Bernard AE, Anolik JH, and Sanz I. 2004. J. Immunol. 172: 4298-4307. (Immunoprecipitation)

Monteith CE, Chelack BJ, Davis WC, and Haines DM. 1996. Can. J. Vet. Res. 60(3): 193-198. (Immunohistochemistry - feline tissue)

Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, and Hill TJ. 1995. J. Histochem. Cytochem. 43:313-320. (Immunohistochemistry - frozen tissue, paraffin embedded tissue)

Domiati-Saad R, Ogle EW, and Justement LB. 1993. J. Immunol. 151: 5936-5947. (in vivo blocking)

Gene Symbol:

PTPRC

Official Gene Full Name:

protein tyrosine phosphatase receptor type C

Target:

PTPRC, PTPRC

Alias Symbols:

LCA, LY5, B220, CD45, L-CA, T200, CD45R, GP180, loc, B220, Cd45, L-CA, Ly-5, T200, CD45R, Lyt-4

NCBI Gene Id:

19264; 5788

Protein Name:

receptor-type tyrosine-protein phosphatase C

Description of Target:

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.

Swissprot Id:

P08575, P06800

Protein Accession #:

NP_002829.3

Nucleotide Accession #:

NM_002838.4

Tissue Tool:

Find tissues and cell lines supported by DNA array analysis to express PTPRC.

RNA Seq:

Find tissues and cell lines supported by RNA-seq analysis to express PTPRC.

品牌介绍

avivasysbio英杰华系统生物学

关键目标家族和独特内容的抗体

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Aviva Systems Biology为我们的客户提供了大量用于研究和诊断应用的高质量抗体。英杰华目前提供针对7,000多种不同蛋白质靶标的抗体，这是因为我们的高通量抗体生产每月会释放200多种新抗体。Aviva的重点是针对转录，表观遗传学和细胞信号传导领域关键靶标的抗体。Aviva的抗体库包含针对许多目标的独特抗体含量，这些目标是其他地方无法买到的。我们将重点放在面向家庭的抗体生产上，从而产生了针对大多数关键蛋白质家族成员的抗体，例如转录因子，转录调节剂，RNA结合蛋白，离子通道和细胞膜靶蛋白。